Active species for Ce(IV)-induced hydrolysis of phosphodiester linkage in cAMP and DNA

Jun Sumaoka, Kenichiro Furuki, Yuki Kojima, Masahiko Shibata, Kimihiko Hirao, Naoya Takeda, Makoto Komiyama*

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The hydrolysis of cyclic adenosine 3′,5′-monophosphate and 2′-deoxythymidylyl(3′-5′)2′-deoxythymidine by Ce(NH 4)2(NO3)6 was kinetically studied. The rate of hydrolysis was fairly proportional to the concentration of [Ce 2IV (OH)4]4+, showing that this is the catalytically active species. According to quantum-chemical calculation, the two Ce(IV) ions in this [Ce2IV (OH)4] 4+ cluster are bridged by two OH residues. Upon the complex formation with H2PO4- (a model compound for the phosphodiesters), these two Ce(IV) ions bind the two oxygen atoms of the substrate and enhance the electrophilicity of the phosphorus atom. The catalytic mechanism of Ce(IV)-induced hydrolysis of phosphodiesters has been proposed on the basis these results.

本文言語English
ページ(範囲)523-538
ページ数16
ジャーナルNucleosides, Nucleotides and Nucleic Acids
25
4-6
DOI
出版ステータスPublished - 2006 6 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 遺伝学

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