Activity of the ligand for c-mpl, thrombopoietin, in early haemopoiesis

Ryugo Itoh, Naoyuki Katayama*, Takashi Kato, Nadim Mahmud, Masahiro Masuya, Kohshi Ohishi, Nobuyuki Minami, Horoshi Miyazaki, Horoshi Shiku

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We examined the role of the ligand for c-mpl, thrombopoietin (TPO), in murine early haemopoiesis, using a serum-free culture system. TPO in combination with the ligand for c-kit (SF) or interleukin-3 (IL-3) supported colony formation by marrow cells of 5-fluorouracil (5-FU)-treated mice, whereas TPO alone yielded no colony. When blast cell colonies grown in the presence of TPO plus SF or TPO plus IL-3 were individually replated in suspension cultures containing serum and several growth factors, various combinations of myeloid lineages were seen, indicating that the progenitors supported by TPO plus SF or TPO plus IL-3 are multipotential. Delayed addition experiments demonstrated that TPO has the potential to effectively support the survival of haemopoietic progenitors. We then studied the effects of TPO on proliferative kinetics of cycling progenitors. TPO hastened IL-3-dependent growth of progenitors by shortening the time required for cell cycling. These results suggest that TPO, as a single factor, can support the survival of haemopoietic progenitors and TPO synergizes with SF or IL-3 to act on early multipotential haemopoietic progenitors.

本文言語English
ページ(範囲)228-235
ページ数8
ジャーナルBritish Journal of Haematology
94
2
DOI
出版ステータスPublished - 1996 1 1
外部発表はい

ASJC Scopus subject areas

  • 血液学

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