Adaptive effects of the β2-agonist clenbuterol on expression of β2-adrenoceptor mRNA in rat fast-twitch fiber-rich muscles

Shogo Sato, Sachiko Nomura, Fuuun Kawano, Jun Tanihata, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    研究成果: Article査読

    15 被引用数 (Scopus)

    抄録

    Administration of the β2-agonist clenbuterol has been shown to reduce the expression of β2-adrenoceptor (AR) mRNA in fast-twitch fiber-rich (extensor digitorum longus, EDL) muscle without changing that in slow-twitch fiber-rich (soleus, SOL) muscle in rats. However, the regulatory mechanism for muscle fiber type-dependent down-regulation of the expression of β2-AR mRNA induced by clenbuterol is still unclear. Therefore, mRNA expression of transcriptional and post-transcriptional regulatory factors for β2-AR mRNA levels in fast-twitch fiber-rich (EDL and plantaris, PLA) and slow-twitch fiber-rich (SOL) muscles in clenbuterol-administered (1.0 mg/kg body weight/day for 10 days, subcutaneous) rats was studied by real-time reverse transcription-polymerase chain reaction. Administration of clenbuterol significantly reduced expression of β2-AR mRNA in EDL and PLA muscles without changing that in SOL muscle. Administration of clenbuterol also significantly reduced the mRNA expression of transcriptional regulatory factor (glucocorticoid receptor) and mRNA stabilizing factor (Hu antigen R) in EDL and PLA muscles without changing those in SOL muscle. These results suggest that muscle fiber type-dependent effects of clenbuterol on expression of β2-AR mRNA are closely related to the down-regulation of mRNA expression of transcriptional and post-transcriptional regulatory factors for β2-AR mRNA levels.

    本文言語English
    ページ(範囲)119-127
    ページ数9
    ジャーナルJournal of Physiological Sciences
    60
    2
    DOI
    出版ステータスPublished - 2010 3

    ASJC Scopus subject areas

    • Physiology
    • Medicine(all)

    フィンガープリント 「Adaptive effects of the β<sub>2</sub>-agonist clenbuterol on expression of β<sub>2</sub>-adrenoceptor mRNA in rat fast-twitch fiber-rich muscles」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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