Addition of short guanylyl blocks to oligonucleotide primers with a thermophilic polynucleotide phosphorylase: Its application to the synthesis of oligonucleotides containing guanylyl residues

Yo Kikuchi*, Keiko Hirai, Kenji Sakaguchi

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Polynucleotide phosphorylase from Thermus thermophilus catalyzed the addition of short guanylyl blocks from GDP to the 3'-hydroxyI termini of oligonucleotide primers at low temperature in a simple reaction mixture. Polyguanylic acid formation was inhibited at 37°C, but the addition of one or two guanylyl residues to oligonucleotide primers proceeded in high yields. The reaction was applied to the synthesis of oligonucleotides containing guanylyl residues at the 3'-end. Using (Ap)2A and (Up)2U as primers, (Ap)3G, (Ap)3GpG, and (Up)2G were synthesized in yields of 25-52%. (Ap)2GpG was synthesized from ApA and GDP in a yield of 13%.

本文言語English
ページ(範囲)1427-1432
ページ数6
ジャーナルJournal of Biochemistry
86
5
出版ステータスPublished - 1979 11
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 放射線学、核医学およびイメージング
  • 分子生物学
  • 生化学

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