Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift

Hisanori Wakamatsu, Satomi Takahashi, Takahiro Moriya, Shin Ichi T Inouye, Hitoshi Okamura, Masashi Akiyama, Shigenobu Shibata

    研究成果: Article

    85 引用 (Scopus)

    抄録

    It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.

    元の言語English
    ページ(範囲)127-131
    ページ数5
    ジャーナルNeuroReport
    12
    発行部数1
    出版物ステータスPublished - 2001 1 22

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    Antisense Oligonucleotides
    Light
    Messenger RNA
    Suprachiasmatic Nucleus
    Microinjections
    Circadian Rhythm
    Injections
    Genes

    ASJC Scopus subject areas

    • Neuroscience(all)

    これを引用

    Wakamatsu, H., Takahashi, S., Moriya, T., Inouye, S. I. T., Okamura, H., Akiyama, M., & Shibata, S. (2001). Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift. NeuroReport, 12(1), 127-131.

    Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift. / Wakamatsu, Hisanori; Takahashi, Satomi; Moriya, Takahiro; Inouye, Shin Ichi T; Okamura, Hitoshi; Akiyama, Masashi; Shibata, Shigenobu.

    :: NeuroReport, 巻 12, 番号 1, 22.01.2001, p. 127-131.

    研究成果: Article

    Wakamatsu, H, Takahashi, S, Moriya, T, Inouye, SIT, Okamura, H, Akiyama, M & Shibata, S 2001, 'Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift', NeuroReport, 巻. 12, 番号 1, pp. 127-131.
    Wakamatsu H, Takahashi S, Moriya T, Inouye SIT, Okamura H, Akiyama M その他. Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift. NeuroReport. 2001 1 22;12(1):127-131.
    Wakamatsu, Hisanori ; Takahashi, Satomi ; Moriya, Takahiro ; Inouye, Shin Ichi T ; Okamura, Hitoshi ; Akiyama, Masashi ; Shibata, Shigenobu. / Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift. :: NeuroReport. 2001 ; 巻 12, 番号 1. pp. 127-131.
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    abstract = "It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.",
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    AU - Moriya, Takahiro

    AU - Inouye, Shin Ichi T

    AU - Okamura, Hitoshi

    AU - Akiyama, Masashi

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