Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates

Katsuhisa Matsuura, Masanori Wada, Katsuhisa Sakaguchi, Yuki Matsuhashi, Tatsuya Shimizu

研究成果: Article

抄録

Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.

元の言語English
ジャーナルRegenerative Therapy
DOI
出版物ステータスPublished - 2019 1 1

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Stem cells
Shear stress
Induced Pluripotent Stem Cells
Equipment and Supplies
Cellular manufacturing
Suspensions
Agglomeration
Cell Aggregation
Pluripotent Stem Cells
Regenerative Medicine
Cell Count
Technology

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

これを引用

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title = "Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates",
abstract = "Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.",
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AU - Matsuura, Katsuhisa

AU - Wada, Masanori

AU - Sakaguchi, Katsuhisa

AU - Matsuhashi, Yuki

AU - Shimizu, Tatsuya

PY - 2019/1/1

Y1 - 2019/1/1

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AB - Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.

KW - 3D suspension culture

KW - Cell aggregate dissociation device

KW - iPS cell

KW - Taylor couette flow

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