Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates

Katsuhisa Matsuura*, Masanori Wada, Katsuhisa Sakaguchi, Yuki Matsuhashi, Tatsuya Shimizu

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.

本文言語English
ページ(範囲)6-13
ページ数8
ジャーナルRegenerative Therapy
12
DOI
出版ステータスPublished - 2019 12 15

ASJC Scopus subject areas

  • 生体材料
  • 生体医工学
  • 発生生物学

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