Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells

Masamitsu Kanada, Akira Nagasaki, Taro Q.P. Uyeda*


研究成果: Article査読

77 被引用数 (Scopus)


Myosin II-dependent contraction of the contractile ring drives equatorial furrowing during cytokinesis in animal cells. Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces. Here, we show that in the presence of 30 μM blebbistatin, a potent myosin II inhibitor, normal rat kidney (NRK) cells adhering to fibronectin-coated surfaces formed equatorial furrows and divided in a manner strikingly similar to myosin II-null Dictyostelium cells. Such blebbistatin-resistant cytokinesis was absent in partially detached NRK cells and was disrupted in adherent cells if the advance of their polar lamellipodia was disturbed by neighboring cells. Y-27632 (40 μM), which inhibits Rho-kinase, was similar to 30 μM blebbistatin in that it inhibited cytokinesis of partially detached NRK cells but only prolonged furrow ingression in attached cells. In the presence of 100 μM blebbistatin, most NRK cells that initiated anaphase formed tight furrows, although scission never occurred. Adherent HT1080 fibrosarcoma cells also formed equatorial furrows efficiently in the presence of 100 μM blebbistatin. These results provide direct evidence for adhesion-dependent, contractile ring-independent equatorial furrowing in mammalian cells and demonstrate the importance of substrate adhesion for cytokinesis.

ジャーナルMolecular biology of the cell
出版ステータスPublished - 2005 8月

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学


「Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。