TY - JOUR
T1 - Advancement of differentiation of oligodendrocyte progenitor cells by a cascade including protein kinase A and cyclic AMP-response element binding protein
AU - Shiga, Hatsuki
AU - Asou, Hiroaki
AU - Ito, Etsuro
N1 - Funding Information:
H.S. was funded by the 21st Century COE Program (Hokkaido University) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was supported by a Research Fellowship (No. 09924) of the Japan Society for the Promotion of Science for Young Scientists to H.S., and by Grants-in-Aid (Nos. 13210006, 15014201, and 16014201) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to E.I.
PY - 2005/12
Y1 - 2005/12
N2 - A transcription factor, cyclic AMP-response element binding protein (CREB), which is phosphorylated by protein kinases (PKA and PKC), is known to be involved in the regulation of oligodendrocyte differentiation. However, it is still unclear whether protein kinase A (PKA) and protein kinase C (PKC) are used simultaneously or at different time points to phosphorylate CREB in oligodendrocytes and whether CREB phosphorylation advances oligodendrocyte differentiation or vise versa. Our previous experiments have shown that in the differentiation process from immature to mature cells, CREB phosphorylation depends on PKC activity and leads to the progression of differentiation. In order to gain a better understanding of the process of differentiation from progenitor to immature cells, we identified which protein kinase, i.e., PKA or PKC, regulates CREB phosphorylation and we determined whether CREB phosphorylation advances differentiation or the reverse. Our results showed that CREB phosphorylation is principally regulated by PKA activity in progenitor cells but not by PKC activity, and that this phosphorylation advances the differentiation of progenitor cells to immature cells in oligodendrocytes.
AB - A transcription factor, cyclic AMP-response element binding protein (CREB), which is phosphorylated by protein kinases (PKA and PKC), is known to be involved in the regulation of oligodendrocyte differentiation. However, it is still unclear whether protein kinase A (PKA) and protein kinase C (PKC) are used simultaneously or at different time points to phosphorylate CREB in oligodendrocytes and whether CREB phosphorylation advances oligodendrocyte differentiation or vise versa. Our previous experiments have shown that in the differentiation process from immature to mature cells, CREB phosphorylation depends on PKC activity and leads to the progression of differentiation. In order to gain a better understanding of the process of differentiation from progenitor to immature cells, we identified which protein kinase, i.e., PKA or PKC, regulates CREB phosphorylation and we determined whether CREB phosphorylation advances differentiation or the reverse. Our results showed that CREB phosphorylation is principally regulated by PKA activity in progenitor cells but not by PKC activity, and that this phosphorylation advances the differentiation of progenitor cells to immature cells in oligodendrocytes.
KW - CREB
KW - Oligodendrocyte
KW - PKA
KW - PKC
KW - Phosphorylation
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U2 - 10.1016/j.neures.2005.09.004
DO - 10.1016/j.neures.2005.09.004
M3 - Article
C2 - 16198437
AN - SCOPUS:27844511488
VL - 53
SP - 436
EP - 441
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 4
ER -