Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping

Ken Adachi, Naohiro Noda, Makoto Nakashige, Satoshi Tsuneda, Takahiro Kanagawa*

*この研究の対応する著者

研究成果: Article査読

17 被引用数 (Scopus)

抄録

We have developed a novel method for genotyping based on free solution affinity capillary electrophoresis. We prepared DNA-nanoparticle conjugates by mixing biotin-modified DNA and NeutrAvidin-modified polystyrene nanoparticles; this mixture was then injected into a capillary. Subsequently, we injected the fluorescent-labeled sample DNAs into the capillary, applied the voltage, increased its temperature after 7 min, and detected the fluorescence at its anodic end. This novel method was applied for genotyping human c-K-ras, and the three genotypes were definitely distinguishable with high reproducibility. This method can be easily automated, and it is useful for high-throughput gene mutation analysis.

本文言語English
ページ(範囲)127-131
ページ数5
ジャーナルJournal of Chromatography A
1109
2
DOI
出版ステータスPublished - 2006 3月 24

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学

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