Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle

Zsolt Radak*, Zoltan Bori, Erika Koltai, Ioannis G. Fatouros, Athanasios Z. Jamurtas, Ioannis I. Douroudos, Gerasimos Terzis, Michalis G. Nikolaidis, Athanasios Chatzinikolaou, Apostolos Sovatzidis, Shuzo Kumagai, Hisahi Naito, Istvan Boldogh

*この研究の対応する著者

研究成果: Article査読

73 被引用数 (Scopus)

抄録

8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent.

本文言語English
ページ(範囲)417-423
ページ数7
ジャーナルFree Radical Biology and Medicine
51
2
DOI
出版ステータスPublished - 2011 7 15
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学(医学)

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