Albumin permeability across endothelial monolayers under long-term shear stress

Susumu Kudo*, Masanori Tsuzaka, Mariko Ikeda, Kazuo Tanishita

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Endothelial cells lining the inner surface of blood vessels regulate the exchange of molecules between the blood and the vessel wall. This study investigates the effect of longterm shear stress on macromolecule permeability across endothelial monolayers. In vitro system to measure transendothelial permeability to tetramethylrhodamine conjugated albumin under shear stress was developed. The temporal variation in albumin permeability under 1 Pa and 4 Pa over 48 h was quantified using a fluorescence spectrophotometer. At 4 Pa, albumin permeability did not show a statistical significant change over 48 h. Under shear stress of 1 Pa, the average albumin permeability between 12 and 30 h was 2.5-fold higher than the average between 0 and 6 h. Under shear stress of 1 Pa, the average albumin permeability between 36 and 48 h was 1.8-fold higher than the average between 12 and 30 h. These results show that both the magnitude of, and the length of exposure to, shear stress regulate transendothelial permeability.

本文言語English
ページ(範囲)419-424
ページ数6
ジャーナルJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
48
4
DOI
出版ステータスPublished - 2006 6月 15
外部発表はい

ASJC Scopus subject areas

  • 産業および生産工学
  • 機械工学

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