Algorithm for the precise detection of single and cluster cells in microfluidic applications

Mathias Girault, Akihiro Hattori, Hyonchol Kim, Kenji Matsuura, Masao Odaka, Hideyuki Terazono, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Recent advances in imaging flow cytometry and microfluidic applications have led to the development of suitable mathematical algorithms capable of detecting and identifying targeted cells in images. In contrast to currently existing algorithms, we herein proposed the identification and reconstruction of cell edges based on original approaches that overcome frequent detection limitations such as halos, noise, and droplet boundaries in microfluidic applications. Reconstructed cells are then discriminated between single cells and clusters of round-shaped cells, and cell information such as the area and location of a cell in an image is output. Using this method, 76% of cells detected in an image had an error <5% of the cell area size and 41% of the image had an error <1% of the cell area size (n = 1,000). The method developed in the present study is the first image processing algorithm designed to be flexible in use (i.e. independent of the size of an image, using a microfluidic droplet system or not, and able to recognize cell clusters in an image) and provides the scientific community with a very accurate imaging algorithm in the field of microfluidic applications.

本文言語English
ページ(範囲)731-741
ページ数11
ジャーナルCytometry Part A
89
8
DOI
出版ステータスPublished - 2016 8月 1
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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