Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms

Soji Morishita, Norio Komatsu, Keita Kirito, Aya H. Koda, Yuji Sekiguchi, Satoshi Tsuneda, Naohiro Noda

    研究成果: Article

    9 引用 (Scopus)

    抄録

    We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

    元の言語English
    ページ(範囲)1632-1636
    ページ数5
    ジャーナルLeukemia Research
    35
    発行部数12
    DOI
    出版物ステータスPublished - 2011 12

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    Competitive Binding
    Alleles
    Costs and Cost Analysis
    Polymerase Chain Reaction
    Neoplasms

    ASJC Scopus subject areas

    • Cancer Research
    • Hematology
    • Oncology

    これを引用

    Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms. / Morishita, Soji; Komatsu, Norio; Kirito, Keita; Koda, Aya H.; Sekiguchi, Yuji; Tsuneda, Satoshi; Noda, Naohiro.

    :: Leukemia Research, 巻 35, 番号 12, 12.2011, p. 1632-1636.

    研究成果: Article

    Morishita, Soji ; Komatsu, Norio ; Kirito, Keita ; Koda, Aya H. ; Sekiguchi, Yuji ; Tsuneda, Satoshi ; Noda, Naohiro. / Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms. :: Leukemia Research. 2011 ; 巻 35, 番号 12. pp. 1632-1636.
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    abstract = "We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.",
    keywords = "Allele burden, Fluorescence quenching, Gene quantification, JAK2V617F mutation, Myeloproliferative neoplasms",
    author = "Soji Morishita and Norio Komatsu and Keita Kirito and Koda, {Aya H.} and Yuji Sekiguchi and Satoshi Tsuneda and Naohiro Noda",
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    AU - Morishita, Soji

    AU - Komatsu, Norio

    AU - Kirito, Keita

    AU - Koda, Aya H.

    AU - Sekiguchi, Yuji

    AU - Tsuneda, Satoshi

    AU - Noda, Naohiro

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    N2 - We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

    AB - We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

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    KW - Fluorescence quenching

    KW - Gene quantification

    KW - JAK2V617F mutation

    KW - Myeloproliferative neoplasms

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