Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms

Soji Morishita, Norio Komatsu, Keita Kirito, Aya H. Koda, Yuji Sekiguchi, Satoshi Tsuneda, Naohiro Noda*

*この研究の対応する著者

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

本文言語English
ページ(範囲)1632-1636
ページ数5
ジャーナルLeukemia Research
35
12
DOI
出版ステータスPublished - 2011 12月

ASJC Scopus subject areas

  • 血液学
  • 腫瘍学
  • 癌研究

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