Amino acids 519-524 of Dictyostelium myosin II form a surface loop that aids actin binding by facilitating a conformational change

Taro Q.P. Uyeda*, Bruce Patterson, Leonardo Mendoza, Yuichi Hiratsuka

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Residues 519-524 of Dictyostelium myosin II form a small surface loop on the actin binding face, and have been suggested to bind directly to actin through high affinity hydrophobic interactions. To test this hypothesis, we have characterized mutant myosins that lack this loop in vivo and in vitro. A mutant myosin in which this loop was replaced by an Ala residue (Δ519-524/+A) was non-functional in vivo. Replacement with a single Gly residue instead of Ala yielded partial function, suggesting that structural flexibility, rather than hydrophobicity, is the key feature of the loop. The in vivo phenotype of the mutant enabled us to identify a number of additional amino acid changes that restore function to the Δ519-524/+A mutation. Intriguingly, many of these, including L596S, were located at some distances away from the 519-524 loop. We have also isolated suppressors for the L596S mutant myosin, which was not functional in vivo. The suppressors for Δ519-524/+A and those for L596S showed complementary charge patterns. In ATPase assays, Δ519-524/+A S1 showed very low activity and little enhancement by actin, whereas L596S S1 was hyper active and displayed enhanced affinity for actin. In motility assays, Δ519-524/+A myosin released actin filaments upon addition of ATP and was unable to support movements. L596S myosin was also inactive, but in this case actin filaments stayed immobile even after the addition of ATP. Transient kinetic measurements demonstrated that Δ519-524/+A S1 is not only slower than wild type to bind actin filaments, but also slower to dissociate from actin filaments. Based on these results, we concluded that the 519-524 loop is not a major actin binding site but aids actin binding by facilitating a critical conformational change.

本文言語English
ページ(範囲)685-795
ページ数111
ジャーナルJournal of Muscle Research and Cell Motility
23
7-8
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 細胞生物学

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