Amphiphilic Polysaccharide Nanogels as Artificial Chaperones in Cell-Free Protein Synthesis

Yoshihiro Sasaki, Wakiko Asayama, Tatsuya Niwa, Shin Ichi Sawada, Takuya Ueda, Hideki Taguchi, Kazunari Akiyoshi*

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Cell-free protein synthesis is a promising technique for the rapid production of proteins. However, the application of the cell-free systems requires the development of an artificial chaperone that prevents aggregation of the protein and supports its correct folding. Here, nanogel-based artificial chaperones are introduced that improve the folding efficiency of rhodanese produced in cell-free systems. Although rhodanese suffers from rapid aggregation, rhodanese was successfully expressed in the presence of the nanogel and folded to the enzymatically active form after addition of cyclodextrin. To validate the general applicability, the cell-free synthesis of ten water-soluble proteins was examined. It is concluded that the nanogel enables efficient expression of proteins with strong aggregation tendency.

本文言語English
ページ(範囲)814-820
ページ数7
ジャーナルMacromolecular Bioscience
11
6
DOI
出版ステータスPublished - 2011 6 14
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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