An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors

Jun Nakanishi*, Kenji Sugiyama, Hirotaka Matsuo, Yo Ko Takahashi, Satoshi O. Mura, Takuji Nakashima

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to celladhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.

本文言語English
ページ(範囲)65-69
ページ数5
ジャーナルAnalytical Sciences
35
1
DOI
出版ステータスPublished - 2019
外部発表はい

ASJC Scopus subject areas

  • 分析化学

フィンガープリント

「An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル