An astrocyte-specific enhacer of the aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements

Yoichiro Abe, Hiroko Ikeshima-Kataoka, Wakami Goda, Takako Niikura, Masato Yasui

研究成果: Article

8 引用 (Scopus)

抄録

Aquaporin-4, a predominant water channel in the brain, is specifically expressed in astrocyte endfeet and plays a central role in water homeostasis, neuronal activity, and cell migration in the brain. It has two dominant isoforms called M1 and M23, whose mRNA is driven by distinct promoters located upstream of exons 0 and 1 of the aquaporin-4 gene, respectively. To identify cis-acting elements responsible for the astrocyte-specific transcription of M1 mRNA, the promoter activity of the 5′-flanking region upstream of exon 0 in primary cultured mouse astrocytes was examined by luciferase assay, and sequences, where nuclear factors bind, were identified by electrophoretic mobility shift assay. An astrocyte-specific activity enhancing transcription from the M1 promoter was observed within ∼2 kb from the transcriptional start sites of M1 mRNA. At least five elements clustered within the 286-bp region were found to function as a novel astrocyte-specific enhancer. Among the five elements, a consensus sequence of Pit-1/Oct/Unc-86 (POU) transcription factors was indispensable to the astrocyte-specific enhancer since disruption of the POU motif completely abolished the enhancer activity in astrocytes. However, the POU motif alone had little activity, indicating the requirement for cooperation with other upstream elements to exert full enhancer activity.

元の言語English
ページ(範囲)899-912
ページ数14
ジャーナルJournal of Neurochemistry
120
発行部数6
DOI
出版物ステータスPublished - 2012 3
外部発表Yes

Fingerprint

Aquaporin 4
Consensus Sequence
Astrocytes
Transcription Factors
Genes
Transcription
Messenger RNA
Exons
Assays
Brain
Electrophoretic mobility
Aquaporins
5' Flanking Region
Electrophoretic Mobility Shift Assay
Luciferases
Cell Movement
Protein Isoforms
Homeostasis
Water

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

これを引用

An astrocyte-specific enhacer of the aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements. / Abe, Yoichiro; Ikeshima-Kataoka, Hiroko; Goda, Wakami; Niikura, Takako; Yasui, Masato.

:: Journal of Neurochemistry, 巻 120, 番号 6, 03.2012, p. 899-912.

研究成果: Article

Abe, Yoichiro ; Ikeshima-Kataoka, Hiroko ; Goda, Wakami ; Niikura, Takako ; Yasui, Masato. / An astrocyte-specific enhacer of the aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements. :: Journal of Neurochemistry. 2012 ; 巻 120, 番号 6. pp. 899-912.
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