An "Elongated" Translation Elongation Factor Tu for Truncated tRNAs in Nematode Mitochondria

Takashi Ohtsuki, Yoh Ichi Watanabe, Chie Takemoto, Gota Kawai, Takuya Ueda, Kiyoshi Kita, Somei Kojima, Yoshito Kaziro, Jens Nyborg, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

51 被引用数 (Scopus)

抄録

We have found the gene for a translation elongation factor Tu (EF-Tu) homologue in the genome of the nematode Caenorhabditis elegans. Because the corresponding protein was detected immunologically in a nematode mitochondrial (mt) extract, it could be regarded as a nematode mt EF-Tu. The protein possesses an extension of about 57 amino acids (we call this domain 3′) at the C terminus, which is not found in any other known EF-Tu. Because most nematode mt tRNAs lack a T stem, domain 3′ may be related to this feature. The nematode EF-Tu bound to nematode T stem-lacking tRNA, but bacterial EF-Tu was unable to do so. A series of domain exchange experiments strongly suggested that domains 3 and 3′ are essential for binding to T stem-lacking tRNAs. This finding may constitute a novel example of the co-evolution of a structurally simplified RNA and the cognate RNA-binding protein, the latter having apparently acquired an additional domain to compensate for the lack of a binding site(s) on the RNA.

本文言語English
ページ(範囲)21571-21577
ページ数7
ジャーナルJournal of Biological Chemistry
276
24
DOI
出版ステータスPublished - 2001 6月 15
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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