An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa, Takeshi Kano, Chiharu Taga, Masahira Hattori, Yoshiyuki Sakaki, Harukazu Suzuki

研究成果: Article

4 引用 (Scopus)

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We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

元の言語English
ページ(範囲)303-308
ページ数6
ジャーナルFEBS Letters
325
発行部数3
DOI
出版物ステータスPublished - 1993 7 5
外部発表Yes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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