An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa, Takeshi Kano, Chiharu Taga, Masahira Hattori, Yoshiyuki Sakaki, Harukazu Suzuki

研究成果: Article査読

5 被引用数 (Scopus)

抄録

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

本文言語English
ページ(範囲)303-308
ページ数6
ジャーナルFEBS Letters
325
3
DOI
出版ステータスPublished - 1993 7 5
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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