An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes

Dong Sun Kim, Jee Eun Park, Jang Kyoo Shin*, Pan Kyeom Kim, Geunbae Lim, Shuichi Shoji

*この研究の対応する著者

研究成果: Article査読

77 被引用数 (Scopus)

抄録

In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin-biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system (μ-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration.

本文言語English
ページ(範囲)488-494
ページ数7
ジャーナルSensors and Actuators, B: Chemical
117
2
DOI
出版ステータスPublished - 2006 10 12

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

フィンガープリント

「An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル