An in vitro circadian rhythm of protein synthesis in the rat suprachiasmatic nucleus under tissue culture conditions

Shigenobu Shibata*, Toshiyuki Hamada, Keiko Tominaga, Shigenori Watanabe

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Because inhibitors of protein synthesis produce changes in the circadian rhythm of wheel-running activity in rodents, we examined the circadian changes of in vitro protein synthesis by the rat suprachiasmatic nucleus (SCN). We demonstrated a robust circadian rhythm of [14C]leucine incorporation as well as [3H]2-deoxyglucose (2DG) uptake by the SCN. The peak time of 2DG uptake was around circadian time 6-9 h (CT6-CT9). In contrast to 2DG uptake, the maximum rate of leucine incorporation occurred around CT22-CT0. Thus, the leucine incorporation rate preceded 2DG uptake by 6-9 h. Leucine incorporation was inhibited by protein synthesis inhibitors, but not by tetrodotoxin. Since a robust circadian rhythm of leucine incorporation by the SCN was detected in vitro by using our method, this procedure may be useful to study the circadian clock function of the SCN.

本文言語English
ページ(範囲)251-256
ページ数6
ジャーナルBrain Research
584
1-2
DOI
出版ステータスPublished - 1992 7 3
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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