An increase in circulating mast cell colony-forming cells in asthma

H. H. Mwamtemi, K. Koike*, T. Kinoshita, S. Ito, S. Ishida, Y. Nakazawa, Y. Kurokawa, K. Shinozaki, K. Sakashita, K. Takeuchi, M. Shiohara, T. Kamijo, Y. Yasui, A. Ishiguro, Y. Kawano, K. Kitano, H. Miyazaki, T. Kato, S. Sakuma, A. Komiyama

*この研究の対応する著者

研究成果: Article査読

40 被引用数 (Scopus)

抄録

We compared a potential to generate mast cells among various sources of CD34+ peripheral blood (PB) cells in the presence of stem cell factor (SCF) with or without thrombopoietin (TPO), using a serum-deprived liquid culture system. From the time course of relative numbers of tryptase-positive and chymase-positive cells in the cultured cells grown by CD34+ PB cells of nonasthmatic healthy individuals treated with G-CSF, TPO appears to potentiate the SCF-dependent growth of mast cells without influencing the differentiation into mast cell lineage. CD34+ PB cells from asthmatic patients in a stable condition generated significantly more mast cells under stimulation with SCF alone or SCF+TPO at 6 wk of culture than did steady-state CD34+ PB cells of normal controls. Single-cell culture studies showed a substantial difference in the number of SCF-responsive or SCF+TPO-responsive mast cell progenitors in CD34+ PB cells between the two groups. In the presence of TPO, CD34+ PB cells from asthmatic children could respond to a suboptimal concentration of SCF to a greater extent, compared with the values obtained by those of normal controls. Six-week cultured mast cells of asthmatic subjects had maturation properties (intracellular histamine content and tryptase/chymase enzymatic activities) similar to those derived from mobilized CD34+ PB cells of nonasthmatic subjects. An increase in a potential of circulating hemopoietic progenitors to differentiate into mast cell lineage may contribute to the recruitment of mast cells toward sites of asthmatic mucosal inflammation.

本文言語English
ページ(範囲)4672-4677
ページ数6
ジャーナルJournal of Immunology
166
7
DOI
出版ステータスPublished - 2001 4月 1
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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