An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1

C. Nakamura, J. G. Burgess, K. Sode, Tadashi Matsunaga

研究成果: Article査読

140 被引用数 (Scopus)

抄録

Magnetospirillum sp. AMB-1 is a freshwater magnetic bacterium which synthesizes intracellular particles of magnetite (Fe3O4). A genomic DNA fragment required for synthesis of magnetic particles was previously isolated from a nonmagnetic transposon Tn5 mutant. We have determined the complete nucleotide sequence of this fragment. The 2975-base pair region contains two putative open reading frames. One open reading frame, designated magA, encodes a polypeptide which is homologous to the cation efflux proteins, the Escherichia coli potassium ion-translocating protein, KefC, and the putative Na+/H+-antiporter, NapA, from Enterococcus hirae. Northern hybridization demonstrated that the magA mRNA transcript is 1.3 kilobases in size, corresponding to the size of the magA gene. A functional promoter was located upstream from the magA gene, and the transcription in AMB-1 was regulated by environmental iron concentration. Vesicles isolated from E. coli in which the MagA protein was expressed exhibited iron accumulation ability. We consider that the MagA protein is an iron transport involved in the synthesis of magnetic particles in AMB-1.

本文言語English
ページ(範囲)28392-28396
ページ数5
ジャーナルJournal of Biological Chemistry
270
47
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • Biochemistry

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