An observation method for autonomous signaling- mediated synthetic diversification in escherichia coli

Ryoji Sekine, Shotaro Ayukawa, Daisuke Kiga

研究成果: Article査読

抄録

Phenotypic diversification of cells in development and regeneration is conceptually modeled by the motion of marbles rolling down valleys on the Waddington landscape, the main feature of which is bifurcations of the valleys. We have experimentally shown that this feature is sufficient to achieve phenotypic diversification by the construction of a synthetic phenotypic diversification system in Escherichia coli . Cells containing the synthetic phenotypic diversification system were diversified into two distinct cell states, high and low, through autonomous signaling-mediated bifurcation, when all cells were initialized to the low state. In this chapter, we illustrate the detailed experimental procedures involved in the initialization of cells and the observation of the phenotypic diversification.

本文言語English
ページ(範囲)69-74
ページ数6
ジャーナルMethods in Molecular Biology
1151
DOI
出版ステータスPublished - 2014 1 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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