抄録
Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.
本文言語 | English |
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ページ(範囲) | 17-27 |
ページ数 | 11 |
ジャーナル | Methods in Molecular Biology |
巻 | 1162 |
DOI | |
出版ステータス | Published - 2014 |
外部発表 | はい |
ASJC Scopus subject areas
- 分子生物学
- 遺伝学
- 医学(全般)