Analysis of DNA endoreplication in the brain neurons in the terrestrial slug, Limax valentianus.

R. Matsuo, Miki Yamagishi, E. Ito

研究成果: Article査読

3 被引用数 (Scopus)

抄録

DNA endoreplication is the DNA synthesis without cell division, resulting in the generation of a nucleus containing a larger amount of genomic DNA compared to a normal diploid genome. There are many such giant neurons in the molluscan brain that are generated as a result of repeated endoreplication. However, it has been controversial whether the endoreplication is the whole genome replication (polyploidy) or the local amplification of the genes that are necessary for the neuron's function (polyteny/polysomy). Here in this study, we investigated these two possibilities by (1) immunohistochemical analysis of the distribution of 5'-bromodeoxyuridine incorporated into the nuclei of the brain neurons, and by (2) quantitative genomic PCR directed to two different genes expressed in specific brain regions. Our data supported the view that the DNA endoreplication is the whole genome replication rather than the local amplification of a specific genomic region.

本文言語English
ページ(範囲)171-178
ページ数8
ジャーナルActa biologica Hungarica
63 Suppl 2
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 環境科学(全般)
  • 神経学

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