抜粋
Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.
| 元の言語 | English |
|---|---|
| ホスト出版物のタイトル | TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems |
| 出版者 | Institute of Electrical and Electronics Engineers Inc. |
| ページ | 634-637 |
| ページ数 | 4 |
| ISBN（電子版） | 9781538627310 |
| DOI | |
| 出版物ステータス | Published - 2017 7 26 |
| イベント | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China 継続期間: 2017 6 18 → 2017 6 22 |
Other
| Other | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 |
|---|---|
| 国 | Taiwan, Province of China |
| 市 | Kaohsiung |
| 期間 | 17/6/18 → 17/6/22 |
ASJC Scopus subject areas
- Chemical Health and Safety
- Instrumentation
- Electrical and Electronic Engineering
フィンガープリント Analysis of environmental bacteria at single-cell level' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。
これを引用
Hosokawa, M., Nishikawa, Y., Kogawa, M., & Takeyama, H. (2017). Analysis of environmental bacteria at single-cell level. ： TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 634-637). [7994129] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994129