Analysis of environmental bacteria at single-cell level

Masahito Hosokawa; Yohei Nishikawa; Masato Kogawa; Haruko Takeyama

doi:10.1109/TRANSDUCERS.2017.7994129

Analysis of environmental bacteria at single-cell level

Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama

研究成果: Conference contribution

抄録

Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.

本文言語	English
ホスト出版物のタイトル	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
出版社	Institute of Electrical and Electronics Engineers Inc.
ページ	634-637
ページ数	4
ISBN（電子版）	9781538627310
DOI	https://doi.org/10.1109/TRANSDUCERS.2017.7994129
出版ステータス	Published - 2017 7 26
イベント	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China 継続期間: 2017 6 18 → 2017 6 22

Other

Other	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country	Taiwan, Province of China
City	Kaohsiung
Period	17/6/18 → 17/6/22

ASJC Scopus subject areas

Chemical Health and Safety
Instrumentation
Electrical and Electronic Engineering

文献へのアクセス

10.1109/TRANSDUCERS.2017.7994129

フィンガープリント「Analysis of environmental bacteria at single-cell level」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Hosokawa, M, Nishikawa, Y, Kogawa, M & Takeyama, H 2017, Analysis of environmental bacteria at single-cell level. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994129, Institute of Electrical and Electronics Engineers Inc., pp. 634-637, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 17/6/18. https://doi.org/10.1109/TRANSDUCERS.2017.7994129

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