Analysis of ferrylHb radical reduction by L-tyrosine and its application to hemoglobin vesicles

Tomovasu Atoji, Hirotaka Yatami, Motonari Aihara, Eishun Tsuchida, Shinji Takeoka

研究成果: Paper査読

抄録

We found that a mixed solution of methemoglobin (metHb) and L-tyrosine (L-Tyr) showed an effective H2O2 elimination ability by utilizing the peroxidase activity of metHb using L-Tyr as a substrate. The rapid radical disappearance and the appearance of metHb signal by L-Tyr addition were confirmed in the ESR measurement. In the mechanism, ferrylHb radicals are reduced to metHb by the electron donation from L-Tyr. From these results, stable H2O2 elimination by metHb/L-Tyr mixed solution would be due to the combination of peroxidase and pseudo-catalase activity of ferrylHb radicals. Furthermore, in the application to the Hb vesicles co-encapsulating metHb/L-Tyr, the 50 % metHb conversion from oxyHb in the vesicles was prolonged to 44 hr from 14hr of the Hb vesicles without metHb/L-Tyr.

本文言語English
ページ数1
出版ステータスPublished - 2006 10 19
イベント55th SPSJ Annual Meeting - Nagoya, Japan
継続期間: 2006 5 242006 5 26

Conference

Conference55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

ASJC Scopus subject areas

  • Engineering(all)

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