Analysis of histidine-dependent antitermination in Bacillus subtilis hut operon.

M. Oda*, Naoto Kobayashi, Y. Kurusu, M. Fujita

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We have previously shown that a positive regulator, HutP, of Bacillus subtilis hut operon is a RNA binding protein. Here, we report precise binding site of HutP in cis-regulatory region on hut mRNA and the role of HutP in histidine-dependent antitermination of hut expression. Ethylnitrosourea modification interference assay showed that four binding sites of HutP were found in the cis-regulatory sequences and were located at the stem and the internal loop of an antiterminator structure. In vitro transcription assay indicated that HutP suppressed transcription termination in the presence of histidine. These results suggest that HutP function as an antiterminator in response to the presence of histidine.

本文言語English
ページ(範囲)5-6
ページ数2
ジャーナルNucleic acids symposium series
44
出版ステータスPublished - 2000
外部発表はい

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