Analysis of individual signaling complexes by mMAPS, a flow-proteometric system

Chao Kai Chou, Pei Hsiang Tsou, Jennifer L. Hsu, Heng Huan Lee, Ying Nai Wang, Jun Kameoka, Mien Chie Hung

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Signal transduction is essential for maintaining normal cell physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for multiparameter analysis of proteins in a single complex (mMAPS) for analyzing and quantifying individual target signaling complexes. mMAPS is a flow-proteometric system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissues or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe and requiring very low sample input.

本文言語English
論文番号20.11
ジャーナルCurrent Protocols in Molecular Biology
2016
DOI
出版ステータスPublished - 2016 4月 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学

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