Analysis of the contraction of fibroblast-collagen gels and the traction force of individual cells by a novel elementary structural model

Z. Feng*, Y. Wagatsuma, S. Kobayashi, T. Kosawada, D. Sato, T. Nakamura, T. Kitajima, M. Umezu

*この研究の対応する著者

研究成果: Conference contribution

5 被引用数 (Scopus)

抄録

Based on the experimental data of the contraction ratio of fibroblast-collagen gels with different initial collagen concentrations and cell numbers, we analyzed the traction force exerted by individual cells through a novel elementary structural model. We postulate that the mechanical mechanism of the gel contraction is mainly because that populated cells apply traction force to some of the surrounding collagen fibrils with such proper length potential to be pulled straight so as to be able to sustain the traction force; this traction induce the cells moving closely to each other and consequently compact the fibrillar network; the bending force of the fibrils in turn resists the movement. By employing fiber packing theory for random fibrillar networks and network alteration theory, the bending force of collagen fibrils was deduced. The traction force exerted by individual fibroblasts in the gels was balanced by the bending force and the resistance from interstitial fluid since inertial force can be neglected. The maximum traction force per cell under free floating condition is in the range of 0.27-9.02 nN depending on the initial collagen concentration and populated cell number. The most important outcome of this study is that the traction force of individual cells dynamically varies under different gel conditions, whereas the adhesion force between cell and individual fibrils is relatively converging and stable.

本文言語English
ホスト出版物のタイトル2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
ページ6232-6235
ページ数4
DOI
出版ステータスPublished - 2013 10月 31
イベント2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan
継続期間: 2013 7月 32013 7月 7

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
国/地域Japan
CityOsaka
Period13/7/313/7/7

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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