Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors

Kenta Akama, Brooke E. Christian, Christie N. Jones, Takuya Ueda, Nono Takeuchi*, Linda L. Spremulli


研究成果: Article査読

11 被引用数 (Scopus)


Mammalian mitochondria synthesize a set of thirteen proteins that are essential for energy generation via oxidative phosphorylation. The genes for all of the factors required for synthesis of the mitochondrially encoded proteins are located in the nuclear genome. A number of disease-causing mutations have been identified in these genes. In this manuscript, we have elucidated the mechanisms of translational failure for two disease states characterized by lethal mutations in mitochondrial elongation factor Ts (EF-Tsmt) and elongation factor Tu (EF-Tumt). EF-Tumt delivers the aminoacyl-tRNA (aa-tRNA) to the ribosome during the elongation phase of protein synthesis. EF-Tsmt regenerates EF-Tumt:GTP from EF-Tumt:GDP. A mutation of EF-Tsmt (R325W) leads to a two-fold reduction in its ability to stimulate the activity of EF-Tumt in poly(U)-directed polypeptide chain elongation. This loss of activity is caused by a significant reduction in the ability of EF-Tsmt R325W to bind EF-Tumt, leading to a defect in nucleotide exchange. A mutation of Arg336 to Gln in EF-Tumt causes infantile encephalopathy caused by defects in mitochondrial translation. EF-Tumt R336Q is as active as the wild-type protein in polymerization using Escherichia coli 70S ribosomes and E. coli [14C]Phe-tRNA but is inactive in polymerization with mitochondrial [14C]Phe-tRNA and mitochondrial 55S ribosomes. The R336Q mutation causes a two-fold decrease in ternary complex formation with E. coli aa-tRNA but completely inactivates EF-Tumt for binding to mitochondrial aa-tRNA. Clearly the R336Q mutation in EF-Tumt has a far more drastic effect on its interaction with mitochondrial aa-tRNAs than bacterial aa-tRNAs.

ジャーナルBiochimica et Biophysica Acta - Molecular Basis of Disease
出版ステータスPublished - 2010 7月

ASJC Scopus subject areas

  • 分子医療
  • 分子生物学


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