Androgen receptor of the frog Rana rugosa: Molecular cloning and its characterization

Satoshi Yokoyama, Yuki Oshima, Junpei Tokita, Mari Suda, Takuma Shinozuka, Masahisa Nakamura

    研究成果: Article

    24 引用 (Scopus)

    抄録

    The androgen receptor(AR) gene is located on the Z and W sex chromosomes in the frog Rana rugosa, designated Z- and W-AR, respectively. Among various tissues of an adult frog, AR expression levels were highest in the testis and brain. In the testis, AR was expressed in germ cells. AR expression occured in developing embryos from stage 21 and was very high in the gonad of a male tadpole before the onset of sex determination. When Z- and W-AR were expressed in Xenopus A6 cells, they activated androgen-dependent transcription of a luciferase reporter gene. By contrast, estrogen receptor (ER) a and b showed no sexually dimorphic expression during sex determination, but their expressions became much higher in the gonad of a female tadpole after sex determination. In addition, AR transcripts in the ZZ-tadpoles were twice as abundant as in the ZW genotype. In contrast, W-AR expression was extremely low although when W-AR was expressed in A6 cells, it activated transcription in the luciferase assay. In this regard it is worth noting that the promoter regions of Z- and W-AR are not identical. The results suggest that Z-AR plays an important role in the testis formation in a R. rugosa tadpole, whereas ERb is involved in ovary differentiation. Very low expression of W-AR may be due to its promoter region having mutations in key transcription factor binding sites, although these remain to be identified. Thus, it is proposed that AR could be a candidate for a male-determining gene in R. rugosa.

    元の言語English
    ページ(範囲)796-812
    ページ数17
    ジャーナルJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
    311
    発行部数10
    DOI
    出版物ステータスPublished - 2009

    Fingerprint

    Ranidae
    androgen
    Androgen Receptors
    Molecular Cloning
    frog
    Anura
    frogs
    molecular cloning
    tadpoles
    Larva
    sex determination
    Testis
    testes
    Gonads
    luciferase
    Luciferases
    Genetic Promoter Regions
    androgen receptors
    Glandirana rugosa
    cloning

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Genetics
    • Physiology
    • Molecular Biology

    これを引用

    Androgen receptor of the frog Rana rugosa : Molecular cloning and its characterization. / Yokoyama, Satoshi; Oshima, Yuki; Tokita, Junpei; Suda, Mari; Shinozuka, Takuma; Nakamura, Masahisa.

    :: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 巻 311, 番号 10, 2009, p. 796-812.

    研究成果: Article

    Yokoyama, Satoshi ; Oshima, Yuki ; Tokita, Junpei ; Suda, Mari ; Shinozuka, Takuma ; Nakamura, Masahisa. / Androgen receptor of the frog Rana rugosa : Molecular cloning and its characterization. :: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2009 ; 巻 311, 番号 10. pp. 796-812.
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    abstract = "The androgen receptor(AR) gene is located on the Z and W sex chromosomes in the frog Rana rugosa, designated Z- and W-AR, respectively. Among various tissues of an adult frog, AR expression levels were highest in the testis and brain. In the testis, AR was expressed in germ cells. AR expression occured in developing embryos from stage 21 and was very high in the gonad of a male tadpole before the onset of sex determination. When Z- and W-AR were expressed in Xenopus A6 cells, they activated androgen-dependent transcription of a luciferase reporter gene. By contrast, estrogen receptor (ER) a and b showed no sexually dimorphic expression during sex determination, but their expressions became much higher in the gonad of a female tadpole after sex determination. In addition, AR transcripts in the ZZ-tadpoles were twice as abundant as in the ZW genotype. In contrast, W-AR expression was extremely low although when W-AR was expressed in A6 cells, it activated transcription in the luciferase assay. In this regard it is worth noting that the promoter regions of Z- and W-AR are not identical. The results suggest that Z-AR plays an important role in the testis formation in a R. rugosa tadpole, whereas ERb is involved in ovary differentiation. Very low expression of W-AR may be due to its promoter region having mutations in key transcription factor binding sites, although these remain to be identified. Thus, it is proposed that AR could be a candidate for a male-determining gene in R. rugosa.",
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