Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

Hiroyuki Nakagawa, Masaaki Yoshiyama, Susumu Shimura, Kohtaro Kirimura, Shoji Usami

研究成果: Article査読

16 被引用数 (Scopus)

抄録

L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C-NMR analysis.

本文言語English
ページ(範囲)1914-1915
ページ数2
ジャーナルBioscience, Biotechnology and Biochemistry
60
11
DOI
出版ステータスPublished - 1996 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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