Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells

M. Inudoh, H. Nyunoya, T. Tanaka, M. Hijikata, N. Kato, K. Shimotohno*

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence. E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the complex form of E2 protein was greater than that of the high-mannose form of E2 protein. This result indicated that the complex form of the E2 protein is superior for use in diagnosing HCV infection.

本文言語English
ページ(範囲)1590-1596
ページ数7
ジャーナルVaccine
14
17-18
DOI
出版ステータスPublished - 1996 12月
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 免疫学および微生物学(全般)
  • 獣医学(全般)
  • 公衆衛生学、環境および労働衛生
  • 感染症

フィンガープリント

「Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル