Antisense transcripts with rice full-length cDNAs.

Naoki Osato*, Hitomi Yamada, Kouji Satoh, Hisako Ooka, Makoto Yamamoto, Kohji Suzuki, Jun Kawai, Piero Carninci, Yasuhiro Ohtomo, Kazuo Murakami, Kenichi Matsubara, Shoshi Kikuchi, Yoshihide Hayashizaki

*この研究の対応する著者

研究成果: Article査読

105 被引用数 (Scopus)

抄録

BACKGROUND: Natural antisense transcripts control gene expression through post-transcriptional gene silencing by annealing to the complementary sequence of the sense transcript. Because many genome and mRNA sequences have become available recently, genome-wide searches for sense-antisense transcripts have been reported, but few plant sense-antisense transcript pairs have been studied. The Rice Full-Length cDNA Sequencing Project has enabled computational searching of a large number of plant sense-antisense transcript pairs. RESULTS: We identified sense-antisense transcript pairs from 32,127 full-length rice cDNA sequences produced by this project and public rice mRNA sequences by aligning the cDNA sequences with rice genome sequences. We discovered 687 bidirectional transcript pairs in rice, including sense-antisense transcript pairs. Both sense and antisense strands of 342 pairs (50%) showed homology to at least one expressed sequence tag other than that of the pair. Microarray analysis showed 82 pairs (32%) out of 258 pairs on the microarray were more highly expressed than the median expression intensity of 21,938 rice transcriptional units. Both sense and antisense strands of 594 pairs (86%) had coding potential. CONCLUSIONS: The large number of plant sense-antisense transcript pairs suggests that gene regulation by antisense transcripts occurs in plants and not only in animals. On the basis of our results, experiments should be carried out to analyze the function of plant antisense transcripts.

本文言語English
ページ(範囲)R5
ジャーナルGenome Biology
5
1
出版ステータスPublished - 2003
外部発表はい

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 遺伝学
  • 細胞生物学

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