Antisera to calreticulin inhibits sperm motility in mice

Masahisa Nakamura*, Shigeru Oshio, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Mouse sperm were rapidly immobilized when exposed to rabbit antisera against rat calreticulin. The inhibition of sperm motility was concentration dependent at dilutions 1:50-350. The velocity of sperm did not change significantly as long as they were motile. Neither motility nor velocity of sperm was affected by adding sheep antisera to bovine calmodulin. The antisera to calreticulin also inhibited in vitro fertilization of mouse eggs. Using the avidin-biotin-peroxidase complex method, the antigen was found to be localized in the acrosome of sperm. The results indicate that calreticulin is present in the acrosome of mouse sperm and may play an important role in sperm motility.

本文言語English
ページ(範囲)984-990
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
186
2
DOI
出版ステータスPublished - 1992 7月 31
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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