Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama, Akira Yamazawa, Chikashi Nakamura, Tadashi Matsunaga

研究成果: Article査読

40 被引用数 (Scopus)

抄録

Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

本文言語English
ページ(範囲)355-360
ページ数6
ジャーナルBiotechnology Techniques
9
5
DOI
出版ステータスPublished - 1995 5 1
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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