Application of consistent massage-like perturbations on mouse calves and monitoring the resulting intramuscular pressure changes

Naoyoshi Sakitani, Takahiro Maekawa, Kumiko Saitou, Katsuhiko Suzuki, Shuhei Murase, Masakuni Tokunaga, Daisuke Yoshino, Keisuke Sawada, Atsushi Takashima, Motoshi Nagao, Toru Ogata, Yasuhiro Sawada*

*この研究の対応する著者

研究成果査読

1 被引用数 (Scopus)

抄録

Massage is generally recognized to be beneficial for relieving pain and inflammation. Although previous studies have reported anti-inflammatory effects of massage on skeletal muscles, the molecular mechanisms behind are poorly understood. We have recently developed a simple device to apply local cyclical compression (LCC), which can generate intramuscular pressure waves with varying amplitudes. Using this device, we have demonstrated that LCC modulates inflammatory responses of macrophages in situ and alleviates immobilization-induced muscle atrophy. Here, we describe protocols for the optimization and application of LCC as a massage-like intervention against immobilization-induced inflammation and atrophy of skeletal muscles of mouse hindlimbs. The protocol that we have developed can be useful for investigating the mechanism underlying beneficial effects of physical exercise and massage. Our experimental system provides a prototype of the analytical approach to elucidate the mechanical regulation of muscle homeostasis, although further development needs to be made for more comprehensive studies.

本文言語English
論文番号e59475
ジャーナルJournal of Visualized Experiments
2019
151
DOI
出版ステータスPublished - 2019

ASJC Scopus subject areas

  • 神経科学(全般)
  • 化学工学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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