Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability

Takashi Tanii*, Kosuke Sasaki, Kota Ichisawa, Takanori Demura, Yuichi Beppu, Hoan Anh Vu, Hoan Thanh Chi, Hideaki Yamamoto, Yuko Sato

*この研究の対応する著者

研究成果: Article査読

8 被引用数 (Scopus)

抄録

The adhesive ability of two human pancreatic cancer cell lines was evaluated using organosilane monolayer templates (OMTs). Using the OMT, the spreading area of adhered cells can be limited, and this enables us to focus on the initial attachment process of adhesion. Moreover, it becomes possible to arrange the cells in an array and to quantitatively evaluate the number of attached cells. The adhesive ability of the cancer cells cultured on the OMT was controlled by adding (-)-epigallocatechin-3-gallate (EGCG), which blocks a receptor that mediates cell adhesion and is overexpressed in cancer cells. Measurement of the relative ability of the cancer cells to attach to the OMT revealed that the ability for attachment decreased with increasing EGCG concentration. The results agreed well with the western blot analysis, indicating that the OMT can potentially be employed to evaluate the adhesive ability of various cancer cells.

本文言語English
論文番号06GL01
ジャーナルJapanese journal of applied physics
50
6 PART 2
DOI
出版ステータスPublished - 2011 6

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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