Approaches for improving protein production by cell surface engineering

Takeko Kodama, Kenji Manabe, Katsutoshi Ara, Junichi Sekiguchi

研究成果: Chapter

抄録

Bacillus subtilis is an attractive host organism because it has a naturally high secretory capacity and exports proteins directly into the extracellular medium. Secreted proteins emerge from translocation to the compartment between the cell wall and cytoplasmic membrane. It has been reported that the increased net negative charge of the cell wall is involved in protein folding and stability at the cytoplasmic membrane-cell wall interface of B. subtilis. In Escherichia coli, Sec translocase requires a functional interaction with the membrane acidic lipids such as phosphatidylglycerol (PG) and cardiolipin (CL). In Sect. 13.1, we describe altered compositions of anionic polymers on the cell surface and cell membrane lipid of B. subtilis, which is improved for protein secretion. Inactivation of extracellular proteases is essential for improvement of secreted proteins with B. subtilis as a host. Previously we reported that the decrease of extracellular protease amounts in B. subtilis mutants led to the stabilization of autolysins, making the cells more prone to lysis (Kodama et al., J Biosci Bioeng 103:13-21, 2007). In Sect. 13.2, we describe the improvement process for protein production from the aspect of prevention of cell lysis.

本文言語English
ホスト出版物のタイトルMicrobial Production
ホスト出版物のサブタイトルFrom Genome Design to Cell Engineering
出版社Springer Japan
ページ141-147
ページ数7
ISBN(電子版)9784431546078
ISBN(印刷版)4431546065, 9784431546061
DOI
出版ステータスPublished - 2013 11 1
外部発表はい

ASJC Scopus subject areas

  • 免疫学および微生物学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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