Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii*, Shinya Tajima, Hiroshi Kawarada

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

本文言語English
論文番号27001
ジャーナルApplied Physics Express
4
2
DOI
出版ステータスPublished - 2011 2月

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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