Artificial RNA aptamer production by the marine bacterium Rhodovulum sulfidophilum: Improvement of the aptamer yield using a mutated transcriptional promoter

Hiromichi Suzuki, So Umekage, Terumichi Tanaka, Yo Kikuchi

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Noncoding small RNAs and artificial RNA aptamers are now expected to be potential candidates for RNA therapeutic agents. We previously proposed a unique method for economical production of these RNAs using the marine phototrophic bacterium Rhodovulum sulfidophilum. This bacterium does not produce any ribonucleases but does produce extracellular nucleic acids in the culture medium in nature. Using this bacterium and an engineered plasmid containing the rrn promoter for the RNA expression, we developed a method for production of the streptavidin RNA aptamer in the culture medium. However, the yield of this RNA product in the culture medium by this method was not enough for practical use. In the present paper, we improved the yield of this product by modification of the - 35 region of the rrn promoter so as to escape from the Fis protein control and the use of a new vector plasmid. Using this system, the extracellular RNA aptamer of approximately 200. ng and the total RNA aptamer (both extra- and intracellular form) of about 20 μg from 1. L culture were accomplished by constitutive expression of the gene.

本文言語English
ページ(範囲)458-461
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
112
5
DOI
出版ステータスPublished - 2011 11
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • バイオエンジニアリング

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