Asparagine-linked Glycosylation of the Rat Leukemia Inhibitory Factor Expressed by Simian COS7 Cells

Jun Ichi Aikawa*, Ei Ichiro Sato, Shigeru Kyuwa, Eimei Sato, Ken Sasai, Kunio Shiota, Tomoya Ogawa

*この研究の対応する著者

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The leukemia inhibitory factor (LIF) is a secretory glycoprotein and a pluripotent growth factor which acts in diverse cell systems. LIF has been reported to be heavily glycosylated. In this paper, we examine the transient expression of rat LIF (rLIF) in COS? cells and its glycosylation by a PNGaseF treatment and lectin blot. rLIF expression in COS7 cells resulted in seven molecular species being produced with zero to six N-glycosyl moieties. Mutated rLIF proteins with substitutions at the seven possible N-glycosylation sites were also expressed. An analysis of the molecular weight of the mutated rLIF confirmed the six N-glycosylation sites. Bioassays of mouse leukemia cell lines were performed to analyze the contribution of the glycosyl moieties to their functions. We found that the glycosyl moieties at each of the N-glycosylation sites were not essential to their function of the protein, but the reduced functions to promote the proliferation of DA-1a cells that had been observed for some mutants suggests a biochemical role for the in vitro function.

本文言語English
ページ(範囲)1318-1325
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
62
7
出版ステータスPublished - 1998 7
外部発表はい

ASJC Scopus subject areas

  • 食品科学
  • 生化学、遺伝学、分子生物学(全般)
  • 生化学
  • バイオテクノロジー
  • 化学(その他)
  • 応用微生物学とバイオテクノロジー
  • バイオエンジニアリング

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