Assessment of capacity to capture DNA aerosols by clean filters for molecular biology experiments

Yuki Morono*, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

*この研究の対応する著者

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.

本文言語English
ページ(範囲)222-226
ページ数5
ジャーナルMicrobes and Environments
33
2
DOI
出版ステータスPublished - 2018
外部発表はい

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 土壌科学
  • 植物科学

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