Astrocytic endfeet re-cover blood vessels after removal by laser ablation

Hideaki Kubotera, Hiroko Ikeshima-Kataoka, Yoshiki Hatashita, Anna Letizia Allegra Mascaro, Francesco Saverio Pavone, Takafumi Inoue

    研究成果: Article

    抄録

    The astrocyte, one of the glial cells, plays many functional roles. These include provision of nutrients from blood vessels to neurons, supply of neurotransmitters and support of blood–brain barrier (BBB) integrity. Astrocytes are known to support the integrity of BBB through maintenance of the tight junction between endothelial cells of blood vessels. However, evidence of its direct contribution to BBB is lacking owing to technical limitations. In this study, astrocytic endfeet covering blood vessels were removed by the laser ablation method with two photon laser scanning microscopy in in vivo mouse brain, and the re-covering of blood vessels with the astrocytic endfeet was observed in about half of the cases. Blood vessels kept their integrity without astrocytic endfoot covers: leakage of plasma marker dyes, Evans Blue or dextran-conjugated fluorescein, was not observed from stripped blood vessels, while ablation of vascular walls induced extravasation of Evans Blue. These results suggest that the astrocytic endfeet covering blood vessels do not contribute to the immediate BBB barrier.

    元の言語English
    記事番号1263
    ジャーナルScientific Reports
    9
    発行部数1
    DOI
    出版物ステータスPublished - 2019 12 1

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    Laser Therapy
    Blood Vessels
    Evans Blue
    Astrocytes
    Tight Junctions
    Fluorescein
    Photons
    Confocal Microscopy
    Neuroglia
    Neurotransmitter Agents
    Coloring Agents
    Endothelial Cells
    Maintenance
    Neurons
    Food
    Brain

    ASJC Scopus subject areas

    • General

    これを引用

    Kubotera, H., Ikeshima-Kataoka, H., Hatashita, Y., Allegra Mascaro, A. L., Pavone, F. S., & Inoue, T. (2019). Astrocytic endfeet re-cover blood vessels after removal by laser ablation. Scientific Reports, 9(1), [1263]. https://doi.org/10.1038/s41598-018-37419-4

    Astrocytic endfeet re-cover blood vessels after removal by laser ablation. / Kubotera, Hideaki; Ikeshima-Kataoka, Hiroko; Hatashita, Yoshiki; Allegra Mascaro, Anna Letizia; Pavone, Francesco Saverio; Inoue, Takafumi.

    :: Scientific Reports, 巻 9, 番号 1, 1263, 01.12.2019.

    研究成果: Article

    Kubotera, H, Ikeshima-Kataoka, H, Hatashita, Y, Allegra Mascaro, AL, Pavone, FS & Inoue, T 2019, 'Astrocytic endfeet re-cover blood vessels after removal by laser ablation' Scientific Reports, 巻. 9, 番号 1, 1263. https://doi.org/10.1038/s41598-018-37419-4
    Kubotera H, Ikeshima-Kataoka H, Hatashita Y, Allegra Mascaro AL, Pavone FS, Inoue T. Astrocytic endfeet re-cover blood vessels after removal by laser ablation. Scientific Reports. 2019 12 1;9(1). 1263. https://doi.org/10.1038/s41598-018-37419-4
    Kubotera, Hideaki ; Ikeshima-Kataoka, Hiroko ; Hatashita, Yoshiki ; Allegra Mascaro, Anna Letizia ; Pavone, Francesco Saverio ; Inoue, Takafumi. / Astrocytic endfeet re-cover blood vessels after removal by laser ablation. :: Scientific Reports. 2019 ; 巻 9, 番号 1.
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