Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Hiroyuki Ota, Tae Kyu Lim, Tsuyoshi Tanaka, Tomoko Yoshino, Manabu Harada, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

本文言語English
ページ(範囲)361-368
ページ数8
ジャーナルJournal of Biotechnology
125
3
DOI
出版ステータスPublished - 2006 9 18
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー

フィンガープリント

「Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル