Automation of DNA computing readout method based on real-time PCR implemented on DNA engine opticon 2 system

Muhammad Faiz Mohamed Saaid, Ismail Ibrahim, Shahdan Sudin, Mohd Saberi Mohamad, Zulkifli Md Yusof, Jameel Abdulla Ahmed Mukred, Kamal Khalil, Zuwairie Ibrahim, Junzo Watada

    研究成果: Article査読

    1 被引用数 (Scopus)

    抄録

    Previously, an automation of a DNA computing readout method for the Hamiltonian Path Problem (HPP) has been implemented based on LightCycler System. In this study, a similar readout approach is implemented based on DNA Engine Opticon 2 System. The readout approach consists of two steps: real-time amplification in vitro using TaqMan-based real-time PCR, followed by an in silico phase. The in silico phase consists of a data clustering algorithm and an information processing to extract the Hamiltonian path after the TaqMan \YES and \NO reactions have been identified. The result indicates that the automation of DNA computing readout method can be efficiently implemented on DNA Engine Opticon 2 System.

    本文言語English
    ページ(範囲)1907-1916
    ページ数10
    ジャーナルInternational Journal of Innovative Computing, Information and Control
    8
    3 A
    出版ステータスPublished - 2012 3

    ASJC Scopus subject areas

    • 計算理論と計算数学
    • 情報システム
    • ソフトウェア
    • 理論的コンピュータサイエンス

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