Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles

Atsushi Arakaki, Mami Takahashi, Masahito Hosokawa, Tadashi Matsunaga, Tsuyoshi Tanaka

研究成果: Article

1 引用 (Scopus)

抄録

In this study, a method involving polyamidoamine dendron-modified magnetic nanoparticles (PAMAM-MNPs) along with application of an alternating magnetic field (AMF) was developed for inactivation of bacteria in water samples. The PAMAM-MNPs efficiently bound to Escherichia coli cells, resulting in magnetic recovery of cells from aqueous solutions. By applying the AMF (5kW, 250 kHz) to the cell suspension, E. coli cells were successfully inactivated within 10 min in the presence of the MNPs, while no effect was observed in the absence of the MNPs. The use of PAMAM-MNPs could increase the inactivation rate of E. coli under the applied AMF. E. coli cells with PAMAM-MNPs stained by propidium iodide (PI) exhibited apparent fluorescence after exposure to the AMF, suggesting the occurrence of membrane damage in the cells because of direct heat transfer from the PAMAM-MNPs. Our technique can be used to address bacterial contamination with wide varieties of microorganisms in water samples.

元の言語English
ページ(範囲)324-327
ページ数4
ジャーナルElectrochemistry
84
発行部数5
DOI
出版物ステータスPublished - 2016 5 1
外部発表Yes

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Electromagnetic fields
Escherichia coli
Nanoparticles
Magnetic fields
Water
Propidium
Microorganisms
Suspensions
Bacteria
Contamination
Fluorescence
PAMAM Starburst
dendron
Heat transfer
Membranes
Recovery

ASJC Scopus subject areas

  • Electrochemistry

これを引用

Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles. / Arakaki, Atsushi; Takahashi, Mami; Hosokawa, Masahito; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

:: Electrochemistry, 巻 84, 番号 5, 01.05.2016, p. 324-327.

研究成果: Article

Arakaki, Atsushi ; Takahashi, Mami ; Hosokawa, Masahito ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles. :: Electrochemistry. 2016 ; 巻 84, 番号 5. pp. 324-327.
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