Biochemical analysis of the human DMC1-I37N polymorphism

Juri Hikiba, Yoshimasa Takizawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    The DMC1 protein, a meiosis-specific DNA recombinase, promotes homologous pairing and strand exchange. The I37N single nucleotide polymorphism of the human DMC1 protein was reported as a result of human genome sequencing projects. In this study, we purified the human DMC1-I37N variant, as a recombinant protein. The DMC1 protein is known to require DNA for efficient ATP hydrolysis. By contrast, the DMC1-I37N variant efficiently hydrolyzed ATP in the absence of DNA. Like the conventional DMC1 protein, the DMC1-I37N variant promoted strand exchange, but it required a high Ca2+ concentration (4-8 mm), a condition that inactivates the strand-exchange activity of the conventional DMC1 protein. These biochemical differences between the DMC1 and DMC1-I37N proteins suggest that the DMC1-I37N polymorphism may be a source of improper meiotic recombination, causing meiotic defects in humans.

    本文言語English
    ページ(範囲)457-465
    ページ数9
    ジャーナルFEBS Journal
    276
    2
    DOI
    出版ステータスPublished - 2009 1

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

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